Category: Imaging
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WHY YOU SHOULD ATTEND THE UCSF/QB3 Microscopy Course
Over the course of my career, I’ve flown across the US to attend a variety of imaging related instructional courses, all on the eastern seaboard. We are all familiar with the Woods Hole courses, the AQLM course, and so forth. What has boggled my mind is that here I am in the SF Bay area, […]
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PhotoFluor 2 Driver for Nikon Elements
89 North has asked me to cook up a driver for the PhotoFluor 2, which I am now hosting under my Macros & Journals page, or you can use this link. This driver works in NIS Elements AR, and BR with Advanced Interpreter. Please contact me if you have any trouble using it! -Austin
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Lumencor Spectra Driver Available
I’ve written a Lumencor Spectra Driver that works with NIS Elements B3.2+. (AR or BR + Advanced Interpreter) The driver provides integrated control into elements for both intensity of lines and channel. These appear as a shutter and two filter wheels, offering full integration into Elements’ Optical configurations. We are selling it for $750, including […]
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How to Align a Dualview 2
Walking through the alignment procedure of the DV 2, narrated by Jeff Harris from Photometrics . Great video and great explanation Jeff! For those of you who have a DualView, I’m certain this video will come in handy. httpv://www.youtube.com/watch?v=WJC5_DdaH80
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Nikon Oils, Perfect Focus and What to Use
There are two docs published by Nikon regarding the use of oils. Most people think you can use any old oil for any microscope, but this is not the case. Zeiss, Leica, Nikon and Olympus all have specific chromatic, spherical and viscosity values, which are matched to work with the corrective properties of the lenses. […]
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Acquisition crashes, and power settings in Win7
Windows 7 has a new power management interface, and the design of modern motherboards, along with win7 Automatic Power Management (APM), can cause some insidious device-based software crashes if power options aren’t set correctly. Here’s how to make sure your system is running in the best mode. Open the Windows Control panel Search for and […]
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Voltage output from NIS Elements, MetaMorph or uManager for under $200
I posted briefly about this a while back, in my explanation of the demise of the parallel port. I finally got my hands on a National Instruments USB-6008 box, and have found the unit to be 100% compatible with any NI-DAQ controlling application. What follows is an example of how to set this up using […]
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Nikon C2 – NIS Elements and Spectral Capability
Nikon has redesigned and updates it’s classic C1 Confocal. The C1 was a great core facility confocal as it was a stable and reliable product. The one thing that held it back was the lack of access to NIS Elements software. With the release of the C2 this problem has been solved. So, along […]
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Proper RGB Conversion of Multichannel Images
When acquiring images in NIS Elements, it is possible to acquire each image with a color over that is approximate to the wavelength used for acquisition. This is the easiest way to view and relate multi-channel data, but when converting the images for export to other programs, it cam become a problem. In the image […]
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Determining background sources in fluorescence
For microscopy engineers and suppliers like me, the background produced in an image is of importance, if that background is created by the imaging system itself. What so many of us on this side of the scope (vs the user side) forget to train on and talk about is the specimen side of the background. […]