Tag: ImageJ
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NIS Elements v4 and Bio Formats in Fiji/ImageJ
I’ve been receiving reports that images saved using NIS Elements V4 are not always opening under the typical plugins used for Fiji and/or Image J. If you plan on running a big set of acquisitions I recommend testing a few images before moving forward. In the meantime saving or exporting to TIFF should be a…
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ImageJ Counting Macro for Known Field Values
Here’s a simple method to deal with varying intensities in a large run of images, assuming you have a known number of cells in a field. If I assume I should see, say, more than X cells per area, I can adjust my threshold to slowly increase it’s aggressiveness until that minimum counted value is…
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Merging multiple channels in Fiji
I had this question asked at my recent Bio-X training, and wanted to provide a thorough answer to it, as this is a common need: Assume we have multiple images, or multiple stacks, and we want to analyze something like the intensity through stack A vs. stack B. We don’t want to convert the stacks…