Imaging, Microscopy and More
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What can programming macros do for me?
I’ve been running into a lot of programming applications lately. The fun part about using the Elements (or other) programming system is the amount of utility and flexibility it can offer to end users. Most people think about programming in our industry only as it relates to image analytics. I wanted to point out some…
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Nikon Elements Software v3.0
For anyone using NIS Elements you are probably aware that v3.0 was officially released about 3 months ago. There are a host of new features included in this release and are detailed here. One addition that I’ve been recently very impressed with is the FRET calculation module. This system has changed the approach to the…
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FRET Calculations in Elements 3.0 This summary demonstrates how to calculate FRET crosstalk between samples and how to apply these corrections to a recently acquired FRET sample. Calculation 1. Calibrate FRET Images using samples loaded ONLY with Donor and then ONLY with Acceptor. 2. Open the “Create FRET Image” Window: 3. Load Sequences of acquired…
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Microscopy 101
For the foreseeable future the compound light microscope will continue to be an anchor point in biology, metallurgy, forensics, technology, manufacturing and a host of other fields. Unfortunately it seems lately most educational institutions have reduced the level of instruction on these instruments, while using them has become more complex! In any case I wanted…
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Quick Resolution Calculations
Calibration is something people seem to remember after collecting a bunch of data. There is a simple calculation to use if you need to do a quick calibration or want to figure out a resolution/pixel size trade off. 1. Determine the total magnification of your optical system. This includes: A. The objective magnification B. Any…
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How to Clean Microscopy CCD Camera Windows
Disclaimer: Cleaning the sensor window is a delicate task and you can potentially damage your sensor forever, which most likely will not be covered by the camera manufacturer warranty. Please contact your camera manufacturer to determine what method of cleaning is recommended by them!!! This is a question I run into a lot in the…