FRET Calculations in Elements 3.0 This summary demonstrates how to calculate FRET crosstalk between samples and how to apply these corrections to a recently acquired FRET sample. Calculation 1. Calibrate FRET Images using samples loaded ONLY with Donor and then ONLY with Acceptor. 2. Open the “Create FRET Image” Window: 3. Load Sequences of acquired […]

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Microscopy 101

For the foreseeable future the compound light microscope will continue to be an anchor point in biology, metallurgy, forensics, technology, manufacturing and a host of other fields. Unfortunately it seems lately most educational institutions have reduced the level of instruction on these instruments, while using them has become more complex! In any case I wanted […]

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Quick Resolution Calculations

Calibration is something people seem to remember after collecting a bunch of data. There is a simple calculation to use if you need to do a quick calibration or want to figure out a resolution/pixel size trade off. 1. Determine the total magnification of your optical system. This includes: A. The objective magnification B. Any […]

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How to Clean Microscopy CCD Camera Windows

Disclaimer: Cleaning the sensor window is a delicate task and you can potentially damage your sensor forever, which most likely will not be covered by the camera manufacturer warranty. Please contact your camera manufacturer to determine what method of cleaning is recommended by them!!! This is a question I run into a lot in the […]

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