Lighting Control for Stereo Microscopy

When acquiring images through a stereo microsocpe one of the most common problems to overcome is inconsistant lighting. Whether looking at a printed circuit or looking at a natural specimen such as a fly most of these samples have the potential to reflect light. Add...

Photometrics Releases Evolve EM CCD

A few weeks ago Photometrics released a new EM-CCD camera tagged the Evolve.After attending a webinar presentation on this camera I wanted to point out the major technological benefiets of this system. Time will tell how these additions impact the overall performance...

Elements SP 6 released

Nikon has released SP6 for Elements. There are several improvements for: Ti Microscope controls TIRF controls QImaging cameras ASI stages ND acquisition PFS control in ND acquisitions Macro controls in ND acquisitions There is a complete list of the changes/fixes and...

Stage Search Macro

Stage Scanning Macro for search and focus One of my customers (hi Paul!) asked me to make this macro a while back. Once I got into the design I realized just how useful this would be for basically anyone running an automated stage with Elements. Right-Click and...

Nikon Elements Software v3.0

For anyone using NIS Elements you are probably aware that v3.0 was officially released about 3 months ago. There are a host of new features included in this release and are detailed here. One addition that I’ve been recently very impressed with is the FRET...

FRET Calculations in Elements 3.0 This summary demonstrates how to calculate FRET crosstalk between samples and how to apply these corrections to a recently acquired FRET sample. Calculation 1. Calibrate FRET Images using samples loaded ONLY with Donor and then ONLY...

Microscopy 101

For the foreseeable future the compound light microscope will continue to be an anchor point in biology, metallurgy, forensics, technology, manufacturing and a host of other fields. Unfortunately it seems lately most educational institutions have reduced the level of...

Quick Resolution Calculations

Calibration is something people seem to remember after collecting a bunch of data. There is a simple calculation to use if you need to do a quick calibration or want to figure out a resolution/pixel size trade off. 1. Determine the total magnification of your optical...

How to Clean Microscopy CCD Camera Windows

Disclaimer: Cleaning the sensor window is a delicate task and you can potentially damage your sensor forever, which most likely will not be covered by the camera manufacturer warranty. Please contact your camera manufacturer to determine what method of cleaning is...