Microscope Objective Zemax Files

While I haven’t had a chance to test these for accuracy, I was excited to find them while looking for some tube lens specs! You can find the file set here. These designs include some of the common infinity corrected objectives, as well as tube lenses, from every major microscope manufacturer.    

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How TIRF works on a Microscope

TIRF is a widely used tool for effectively creating a super resolution instrument out of a standard widefield microscope. The cool part of TIRF is that it bypasses the limits of axial resolution on the scope not by altering the optics per-se, but by altering what gets excited. In a normal widefield fluorescent microscope, photons […]

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Coupling a C-2 confocal to an AZ-100 zoom scope

I recently worked on a demo where the customer wanted a confocal with magnification range from macro (1x) to low power compound levels (200-400x). In order to meet this requirement we showed him the AZ-100 zoom scope, and the C-2 scanning confocal, which runs on elements. I was simply blown away by the performance range […]

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Nikon Oils, Perfect Focus and What to Use

There are two docs published by Nikon regarding the use of oils. Most people think you can use any old oil for any microscope, but this is not the case. Zeiss, Leica, Nikon and Olympus all have specific chromatic, spherical and viscosity values, which are matched to work with the corrective properties of the lenses. […]

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Ti-E Perfect Focus Usage and Troubleshooting

The Ti-E PFS is an excellent automatic focusing system. Using the system hinges on one key aspect: The NIR LED used for focusing. The PFS is basically a laser reflection system. A near IR LED is delivered into the back of the objective, and emitted into the specimen. When the light hits a refractive index […]

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